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MASS SPECTROMETRY CORE LABORATORY

Mass Spectrometry is considered the gold standard in drug and metabolite analysis, with the advantages being its ability to detect drugs/metabolites at very low concentrations, its ability to distinguish a specific drug/metabolite from all other compounds in a biologic sample, and its speed, meaning a short turnaround time for results.

Xevo® G2-S QTof

The instrument,  Xevo® G2-S QTof, is designed for scientists who need to identify, quantify, and confirm the broadest range of compounds in the most complex and challenging samples. Incorporating StepWave™ ion optics for unsurpassed levels of durable sensitivity, the Xevo G2-S QTof also uses proven quantitative time-of-flight (QuanTof™) technology to deliver superior UPLC®-compatible mass resolution, matrix tolerant dynamic range, quantitative performance, mass accuracy, and speed of analysis - simultaneously.

This laboratory is currently being set up. Anyone interested in the service may contact Dr. Gang Cheng, associate research professor of pharmaceutical sciences, at (509) 324-7244 or by email at gang8.chen@wsu.edu. This lab will operate the instrument and provide the result to the investigator.

The mass spec is a shared resource of WSU Health Sciences in Spokane.  We gratefully acknowledge the Health Sciences and Services Authority of Spokane County for their financial support to purchase this instrument.

WSUS MICROSCOPY CORE LABORATORY

The WSUS Microscopy Core (WSUS-MC) is a state-of-the-art light microscopy facility and is a shared resource in Medical Sciences at WSU in Spokane that supports WSU and the greater inland Northwest research community.The integrated microscopy core (IMC) is equipped with:

  • Olympus IX-81 Laser Scanning Microscope with multi-color imaging and live cell environmental chamber
  • Zeiss AxioImager M2 upright microscope, for brightfield/darkfield imaging

We gratefully acknowledge the support of the Health Sciences and Services Authority of Spokane County (HSSA) for their financial support to purchase the confocal microscope.

Olympus FluoView1000 Laser Scanning Confocal and IX81 Motorized Inverted Microscope with Deconvolution

The capability of confocal microscopy is performing optical sectioning of thick biologic samples (tissues or cells) and three-dimensional reconstruction of images that are collected.

image of cancer DNA taken with the confocal microscope
Image of cancer DNA taken with the confocal microscope.

Optimized for live-cell imaging applications, the Olympus FluoView1000/IX81 confocal microscope system offers increased stability and brightness needed for long-term live-imaging experiments. Superior frame stability, vibration-free operation, and focus drift compensation make the IX81 well suited for stable observations such as patch clamping. Ultra-precise Z-axis motor with 10nm step size is ideal for optically sectioning during deconvolution or confocal imaging. Four ports offering simultaneous access to a primary image allows spectral separation with multiple detectors for real time, multi-color, co-localization imaging. Additional capabilities include multi-area time lapse, spectral scanning, FRET/FLIP/FRAP, 3D/4D reconstruction, and 3D mosaic imaging.

New Users at WSU Spokane

In order to schedule instrument time you are required to download and complete the New Investigator-Project Form (pdf), including your funding source information. Once this information has been reviewed, you will be contacted by a member of the WSUS-MC staff to discuss the details of your project and schedule your training sessions.To schedule microscope time, please log onto the scope calendar and use the name and password provided to you at your training session.The microscope should be primarily for image acquisition. If you would like to use the computer for image analysis, please keep in mind that in general image acquisition has priority over image analysis

New users from institutions other than WSU Spokane:

Please contact the WSU Spokane microscopy core staff in order to arrange for a meeting so that you may discuss your project with Dr. Weihang Chai or Maria Fadri. Arrangements for scheduling training sessions, appointments, billing, and other matters will be determined at that time.

Microscope Calendar

Please go to calendar.google.com and sign in with the username and password given to you during training.You may sign up for 3 hours per day during peak hours, and for unlimited time during off peak hours.If you need to cancel your appointment, please notify WSUS-MC staff so that your time at the microscope may be freed for other users. 

MICROSCOPY PROTOCOLS


Nuclear Staining with DAPI »

Nuclear Staining with Hoecsht 33342 »

Imaging of Thick Specimens »

Live Imaging of Mitotic Cells »

Live-cell imaging in using heated chamber (pdf) »

Selected protocols posted here from ‘Imaging: A Laboratory Manual’ (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA, 2010. with permission from CSHL press.

MICROSCOPY CORE

Frequently Asked Questions

1. I would like to begin to use the microscope core facilities. How do I start?

New users may contact Weihang Chai (wchai@wsu.edu) or Maria Fadri-Moskwik (m.fadri-moskwik@wsu.edu) to discuss your imaging project and microscope training.

2. What training is required to use the microscope facilities?

Microscope training is comprised of two 2hr practical lessons and is designed to allow users to gain practice and confidence in use of the microscopes at the WSUS-MC. Users may request training on the microscope of their choice.

3. I have completed my training. How do I sign up for time at the microscope?

Users may navigate their web browser to calendar.google.com, log-in using the username and password given to you during training, and then use the drag and drop menus to sign up for time on either the Zeiss fluorescence or Olympus Confocal microscope.

4. I would like to begin to use microscopy for my research project but am new to microscopy. What resources are available to me to help me plan my imaging experiment(s)?

As a starting point, the tutorial, protocol, resource links found on the WSUS-MC website may help provide useful background information on microscopy. WSUS-MC staff are also happy to provide technical assistance. Please contact Weihang Chai (wchai@wsu.edu) or Maria Fadri-Moskwik (m.fadri-moskwik@wsu.edu) for further details.

5. I would like to obtain imaging data but do not want to become a regular microscopy core user. Can I still use the microscope core facilities?

Investigators who would like to use the microscope facilities but do not want to become a regular core user may request assisted imaging time on the microscope of your choice. Please contact Weihang Chai (wchai@wsu.edu) or Maria Fadri-Moskwik (m.fadri-moskwik@wsu.edu) to make arrangements.

CONTACT US

MASS SPECTROMETRY
Dr. Gang Cheng
Pharmaceutical Sciences
(509) 324-7244
gang8.chen@wsu.edu

MICROSCOPY
DIRECTOR

Dr. Weihang Chai
Medical Sciences
wchai@wsu.edu

STAFF
Dr. Maria Fadri-Moskwik
Medical Sciences
m.fradri-moskwik@wsu.edu

Investigators interested in imaging collaborations,
please contact Dr. Chai.

RESOURCES

Mass Spectrometry

Institutional Review Board (IRB) »
Office of Grant and Research Development (OGRD) »

Vivarium forms

CVM ARU Animal Order Form (doc) »

Microscopy

Excitation and Emission Spectra (Invitrogen) »
Excitation and Emission Spectra (University of Arizona) »

NIH Image J Software »