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Microscopy

The WSU Health Sciences Spokane Microscopy Core Laboratory is a state-of-the-art light microscopy facility and is a shared resource at WSU in Spokane that supports the university and the greater Inland Northwest research community.

We are equipped with both widefield and point-scanning confocal microscopes capable of imaging live or fixed cells and tissues.

We gratefully acknowledge the support of the Health Sciences and Services Authority of Spokane County (HSSA) for their financial support to purchase the Olympus IX-81 confocal microscope.

Olympus FluoView1000/IX81 confocal microscope system

The capability of confocal microscopy is performing optical sectioning of thick biologic samples (tissues or cells) and three-dimensional reconstruction of images that are collected. Optimized for live-cell imaging applications, the Olympus FluoView1000 laser scanning confocal and IX81 motorized inverted microscope with deconvolution offers increased stability and brightness needed for long-term live-imaging experiments. Superior frame stability, vibration-free operation, and focus drift compensation make the IX81 well suited for stable observations such as patch clamping. Ultra-precise Z-axis motor with 10nm step size is ideal for optically sectioning during deconvolution or confocal imaging. Four ports offering simultaneous access to a primary image allows spectral separation with multiple detectors for real time, multi-color, co-localization imaging. Additional capabilities include multi-area time lapse, spectral scanning, FRET/FLIP/FRAP, 3D/4D reconstruction, and 3D mosaic imaging.

New WSU Health Sciences Spokane users

To gain access to the microscopy suite in PBS 230N, you will first need to contact Megan Chastain to receive training. Untrained personnel are not permitted in the microscopy suite.

The  Microscopy Core Laboratory is open to both researchers within WSU Health Sciences Spokane and external researchers. Users from outside institutions will need to coordinate with Megan Chastain regarding billing and access prior to using the core laboratory.

New users from other institutions

Please contact Megan Chastain to arrange a meeting to discuss your project. Arrangements for scheduling training sessions, appointments, billing, and other matters will be determined at that time.

Microscope calendar

Please use the LabAgenda Calendar to schedule time in the microscopy CORE. Contact Megan Chastain if you are trained but need help accessing the calendar.

 

MICROSCOPY POLICIES

MICROSCOPY PROTOCOLS

Selected protocols posted here from ‘Imaging: A Laboratory Manual’ (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA, 2010. with permission from CSHL press.

MICROSCOPY TUTORIALS

FREQUENTLY ASKED QUESTIONS »

 

RATES

Note: These rates are currently being evaluated and may increase as of July 1, 2020. Finalized rates will be announced soon. As a reminder, it is best practice to plan for a 3 to 5 percent yearly rate increase when proposing studies and submitting grants.

Microscopy/Histology Imaging Rates (effective Jan. 1, 2019) 
Training$45/hr
Microscopy*Rate
Zeiss M2$20/hr
Zeiss Z1
- Fixed Cell Imaging
- Live Cell Imaging

$20/hr
$30/hr
Olympus IX-81 Confocal
- Fixed Cell Imaging
- Live Cell Imaging

$45/hr
$60/hr
Leica CMi8 Confocal
- Fixed Cell Imaging
- Live Cell Imaging

$45/hr
$60/hr
Image Analysis Computer$10/hr
Technical Support$45/hr