The WSUS Microscopy Core is a state-of-the-art light microscopy facility and is a shared resource in Medical Sciences at WSU in Spokane that supports WSU and the greater inland Northwest research community. The integrated microscopy core (IMC) is equipped with:
- Olympus IX-81 Laser Scanning Microscope with multi-color imaging and live cell environmental chamber
- Zeiss AxioImager M2 upright microscope, for brightfield/darkfield imaging
We gratefully acknowledge the support of the Health Sciences and Services Authority of Spokane County (HSSA) for their financial support to purchase the confocal microscope.
Olympus FluoView1000 Laser Scanning Confocal and IX81 Motorized Inverted Microscope with Deconvolution
The capability of confocal microscopy is performing optical sectioning of thick biologic samples (tissues or cells) and three-dimensional reconstruction of images that are collected. Optimized for live-cell imaging applications, the Olympus FluoView1000/IX81 confocal microscope system offers increased stability and brightness needed for long-term live-imaging experiments. Superior frame stability, vibration-free operation, and focus drift compensation make the IX81 well suited for stable observations such as patch clamping. Ultra-precise Z-axis motor with 10nm step size is ideal for optically sectioning during deconvolution or confocal imaging. Four ports offering simultaneous access to a primary image allows spectral separation with multiple detectors for real time, multi-color, co-localization imaging. Additional capabilities include multi-area time lapse, spectral scanning, FRET/FLIP/FRAP, 3D/4D reconstruction, and 3D mosaic imaging.
New Users at WSU Spokane
In order to schedule instrument time you are required to download and complete the New Investigator-Project Form (pdf), including your funding source information. Once this information has been reviewed, you will be contacted by a member of the WSUS-MC staff to discuss the details of your project and schedule your training sessions. To schedule microscope time, please log onto the scope calendar and use the name and password provided to you at your training session. The microscope should be primarily for image acquisition. If you would like to use the computer for image analysis, please keep in mind that in general image acquisition has priority over image analysis.
New users from institutions other than WSU Spokane:
Please contact the WSU Spokane microscopy core staff in order to arrange for a meeting so that you may discuss your project with Dr. Weihang Chai. Arrangements for scheduling training sessions, appointments, billing, and other matters will be determined at that time.
Please go to calendar.google.com and sign in with the username and password given to you during training.You may sign up for 3 hours per day during peak hours, and for unlimited time during off peak hours.If you need to cancel your appointment, please notify WSUS-MC staff so that your time at the microscope may be freed for other users.
- Nuclear Staining with DAPI »
- Nuclear Staining with Hoecsht 33342 »
- Imaging of Thick Specimens »
- Live Imaging of Mitotic Cells »
- Live-cell imaging in using heated chamber (pdf) »
Selected protocols posted here from ‘Imaging: A Laboratory Manual’ (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA, 2010. with permission from CSHL press.
- Olympus Microscopy Tutorial »
- Nikon Microscopy Tutorial »
- Zeiss Microscopy Tutorial
- Molecular Expressions »
|Zeiss Imager M2 uprght epi-fluorescence||$15/hr||$18/hr||$23/hr|
|Zeiss Observer Z1 inverted epi-fluorescence||$18/hr||$21/hr||$27/hr|
|Image analysis computer (for Zeiss softwares)||$10/hr||$13/hr||$15/hr|
|Zeiss Imager M2 uprght epi-fluorescence||$25/hr||$28/hr||$38/hr|
|Zeiss Observer Z1 inverted epi-fluorescence||$28/hr||$31/hr||$42/hr|
|Image analysis computer (for Zeiss softwares)||$20/hr||$23/hr||$30/hr|
|Training for instrument operation||$30/hr||$40/hr||$60/hr|