{"id":3933,"date":"2020-07-16T00:21:59","date_gmt":"2020-07-16T00:21:59","guid":{"rendered":"https:\/\/spokane.wsu.edu\/research\/?page_id=3933"},"modified":"2025-11-17T16:13:05","modified_gmt":"2025-11-18T00:13:05","slug":"mass-spectometry","status":"publish","type":"page","link":"https:\/\/spokane.wsu.edu\/research\/research-service-centers\/mass-spectometry\/","title":{"rendered":"Mass Spectrometry"},"content":{"rendered":"<div class=\"wsu-container\" >\r\n    \n<div class=\"wsu-decorator wsu-color-background--white wsu-decorator--style-triangle-gray-right\" style=\"bottom:0;left:-50%\"><\/div>\n\n<div class=\"wsu-decorator wsu-color-background--warm-gray wsu-decorator--style-triangle-gray-right\" style=\"bottom:0;left:65%\"><\/div>\n<\/div>\n\n\n<h2 class=\"wp-block-heading  wsu-heading--style-marked\">About the Center<\/h2>\n\n\n<div class=\"wsu-row wsu-row--sidebar-right\" >\r\n    \n<div class=\"wsu-column  wsu-position--relative\"  style=\"\">\r\n\t\n\n<p>Mass spectrometry is a powerful analytical technique for accurately measuring the mass of molecules, identifying unknown compounds within a sample, quantifying known materials, and for elucidating the structure and chemical properties of different molecules.<\/p>\n\n\n\n<p>The Mass Spec Service Center is a WSU Health Sciences shared resource in Spokane, providing state-of-the-art analytical mass spectrometry equipment, analytical services, and expertise in mass spectrometry to researchers from WSU Health Sciences Campus, surrounding academic institutions, and industrial partners. We gratefully acknowledge the Health Sciences and Services Authority of Spokane County for their financial support.<\/p>\n\n<\/div>\r\n\n\n<div class=\"wsu-column\"  style=\"\">\r\n\t\n<article class=\"wsu-card wsu-border-radius--large wsu-dropshadow--medium wsu-spacing-top--xmedium wsu-spacing-bottom--xmedium wsu-spacing-padding-left--xmedium wsu-spacing-padding-right--xmedium wsu-position--relative wsu-overflow--hidden wsu-color-background--warm-gray wsu-border-top--color-light wsu-border--color-crimson-dark wsu-spacing-after--none\">\r\n\t\t<div class=\"wsu-card__content\">\r\n\t\t\t\t\t\t\t\t\t\t<span class=\"wsu-card__content-custom\">\r\n\t\t\t\n<div class=\"wsu-decorator wsu-decorator--style-triangle-gray-right wsu-color-background--gray-30\" style=\"bottom:0;left:55%\"><\/div>\n\n<div class=\"wsu-decorator wsu-color-background--warm-gray wsu-decorator--style-triangle-crimson-right\" style=\"bottom:0;left:80%\"><\/div>\n\n\n<h3 class=\"wp-block-heading\">Service Center Resources<\/h3>\n\n\n\n<ul class=\"wsu-list--style-lined\">\n<li><a href=\"https:\/\/spokane.wsu.edu\/research\/research-service-centers\/mass-spectometry\/policies\/\">Policies<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/spokane.wsu.edu\/research\/documents\/2020\/09\/mass-spectometry-sample-submission-form.pdf\">Sample Submission Form<\/a><\/li>\n<\/ul>\n\n\t\t<\/span>\r\n\t\t\t\t\t<\/div>\r\n\t<\/article>\n<\/div>\r\n\n<\/div>\n\n<section  class=\"wsu-section  wsu-width--full wsu-color-background--transparent\"  >\r\n    <div class=\"wsu-section__inner wsu-width--content\">    \n\n<h2 class=\"wp-block-heading  wsu-heading--style-marked\">Instruments<\/h2>\n\n\n<div class=\"wsu-container\" >\r\n    \n\n<h3 class=\"wp-block-heading\">Xevo\u00ae G2-S QTof<\/h3>\n\n\n<div class=\"wsu-row wsu-row--halves\" >\r\n    \n<div class=\"wsu-column\"  style=\"\">\r\n\t\n\n<p>(Available for full-service analysis by service center staff only)<\/p>\n\n\n\n<p>The Xevo\u00ae G2-S QTof is designed for scientists who need to identify, quantify, and confirm the broadest range of compounds in the most complex and challenging samples. Incorporating StepWave\u2122 ion optics for sensitivity, the Xevo G2-S QTof also uses proven quantitative time-of-flight (QuanTof\u2122) technology to simultaneously deliver superior UPLC\u00ae-compatible mass resolution, matrix tolerant dynamic range, quantitative performance, mass accuracy, and speed of analysis.<\/p>\n\n<\/div>\r\n\n\n<div class=\"wsu-column\"  style=\"\">\r\n\t\n\n<figure class=\"wp-block-image is-resized\"><img decoding=\"async\" loading=\"lazy\" src=\"https:\/\/wpcdn.web.wsu.edu\/wp-spokane\/uploads\/sites\/3493\/2020\/07\/178Xevo\u00ae-G2-S-QTof_300x250px.jpg\" alt=\"Photo of mass spectrometry equipment - 178Xevo\u00ae-G2-S-QTof\" class=\"wsu-border-radius--large wp-image-3928\" width=\"715\" height=\"596\" srcset=\"https:\/\/wpcdn.web.wsu.edu\/wp-spokane\/uploads\/sites\/3493\/2020\/07\/178Xevo\u00ae-G2-S-QTof_300x250px.jpg 300w, https:\/\/wpcdn.web.wsu.edu\/wp-spokane\/uploads\/sites\/3493\/2020\/07\/178Xevo\u00ae-G2-S-QTof_300x250px-150x125.jpg 150w\" sizes=\"(max-width: 715px) 100vw, 715px\" \/><\/figure>\n\n<\/div>\r\n\n<\/div>\n\n\n<h3 class=\"wp-block-heading\">AB Sciex QTRAP 6500 <\/h3>\n\n\n<div class=\"wsu-row wsu-row--halves\" >\r\n    \n<div class=\"wsu-column\"  style=\"\">\r\n\t\n\n<p>(Available on a self-service or full-service basis)<\/p>\n\n\n\n<p>The AB SCIEX QTRAP\u00ae 6500 system merges triple quadrupole and linear ion trap capabilities with new multi-component IonDrive\u2122 technology to provide high sensitivity and wide linear dynamic range for quantitative analysis of analytes with m\/z 5-2000. The AB SCIEX QTRAP\u00ae 6500 is equipped with electrospray (ESI) and atmospheric pressure chemical ionization (APCI). It enables the performance of multiple reaction monitoring (MRM), neutral loss scanning (NL), precursor ion scanning (PIS), NL\/PIS triggered information-dependent acquisition (IDA), and MS\/MS with polarity switching. The QTRAP 6500 is coupled with the Shimadzu Nexera UHPLC system, which provides maximized peak capacity and the lowest carryover for separation of complex biological samples prior to mass spectrometry analysis.<\/p>\n\n<\/div>\r\n\n\n<div class=\"wsu-column\"  style=\"\">\r\n\t\n\n<figure class=\"wp-block-image is-resized\"><img decoding=\"async\" loading=\"lazy\" src=\"https:\/\/wpcdn.web.wsu.edu\/wp-spokane\/uploads\/sites\/3493\/2020\/07\/AB-Sciex-QTRAP-6500_300x250px.jpg\" alt=\"Photo of mass spectrometry equipment - AB-Sciex-QTRAP-6500\" class=\"wsu-border-radius--large wp-image-3929\" width=\"613\" height=\"511\" srcset=\"https:\/\/wpcdn.web.wsu.edu\/wp-spokane\/uploads\/sites\/3493\/2020\/07\/AB-Sciex-QTRAP-6500_300x250px.jpg 300w, https:\/\/wpcdn.web.wsu.edu\/wp-spokane\/uploads\/sites\/3493\/2020\/07\/AB-Sciex-QTRAP-6500_300x250px-150x125.jpg 150w\" sizes=\"(max-width: 613px) 100vw, 613px\" \/><\/figure>\n\n<\/div>\r\n\n<\/div>\n<\/div>\n\n\n<h2 class=\"wp-block-heading  wsu-heading--style-marked\">Services and Fees<\/h2>\n\n\n\n<p>The Mass Spectrometry Service Center may be used on a self-service basis but also provides full-service analysis for small molecules on a fee-for-service basis. This includes accurate mass analysis of small molecules, quantitative measurements of small molecules on the triple quad mass spectrometer using LC-MS\/MS and multiple reaction monitoring (MRM). Other types of analyses include untargeted and targeted metabolomics measurements.<\/p>\n\n\n\n<p>To submit samples, please fill out the <a href=\"https:\/\/spokane.wsu.edu\/research\/documents\/2020\/09\/mass-spectometry-sample-submission-form.pdf\/\">sample submission form (PDF)<\/a> and forward to <a href=\"mailto:ze.liu@wsu.edu\">ze.liu@wsu.edu<\/a>.&nbsp; We are happy to hear from you and consult on any questions related to mass spectrometry.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Rates<\/h3>\n\n\n\n<p>To calculate full-service cost, technical support time is added to machine run time for the applicable instrument. Please note that the Xevo\u00ae G2-S QTof is available on a full-service basis only.<\/p>\n\n\n<div class=\"wsu-note  wsu-note--border-crimson-light\">\n\t\n\t\t\n\t\n\n<p>As a reminder, it is best practice to plan for a 3 to 5 percent yearly rate increase when proposing studies and submitting grants.<\/p>\n\n<\/div>\n\n\n\n<figure class=\"wp-block-table\"><table><thead><tr><th>Service | Equipment<\/th><th>WSU<\/th><th>Non-WSU<\/th><\/tr><\/thead><tbody><tr><td>Xevo\u00ae G2-S QTof<\/td><td>$45\/hr<\/td><td>$50\/hr<\/td><\/tr><tr><td>AB Sciex 6500<\/td><td>$45\/hr<\/td><td>$50\/hr<\/td><\/tr><tr><td>Computer Use Only<\/td><td>$5\/hr<\/td><td>$7.50\/hr<\/td><\/tr><tr><td>Technical Support<\/td><td>$45\/hr<\/td><td>$50\/hr<\/td><\/tr><tr><td>Training<\/td><td>$45\/hr<\/td><td>$50\/hr<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n<div class=\"wsu-note  wsu-note--border-autumn\">\n\t\t\t<i class=\"wsu-note__icon wsu-icon wsu-i-warning-caution\" aria-label=\"Icon label\"><\/i>\n\t\n\t\t\t<div class=\"wsu-note__title\">\n\t\t\tMisuse Fee\t\t<\/div>\n\t\t\n\t\n\n<p>A misuse fee will charge to cover costs for any cleaning or repairs required<\/p>\n\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading  wsu-heading--style-marked\">Training<\/h2>\n\n\n\n<p>The Mass Spectrometry Service Center offers hands-on training in ABSciex 6500 mass spec and Shimadzu Nexera UHPLC system for interested clients.<\/p>\n\n\n\n<p>To schedule a training, please fill out the&nbsp;<a href=\"https:\/\/spokane.wsu.edu\/research\/documents\/2020\/09\/mass-spectometry-training-request-form.pdf\">training request form (PDF)<\/a>&nbsp;and submit it to Mass Spec Service Center supervisor&nbsp;<a href=\"mailto:ze.liu@wsu.edu\">ze.liu@wsu.edu<\/a>.<\/p>\n\n\n\n<h2 class=\"wp-block-heading  wsu-heading--style-marked\">Sample Preparation<\/h2>\n\n\n\n<p>Sample preparation is crucial for obtaining good data with mass spectrometry. Contaminants\u2014including common buffer salts and detergents present in samples\u2014may suppress the ionization of analytes.<\/p>\n\n\n\n<p>If possible, avoid nonvolatile salts (e.g., phosphates, borates, sulfates, or citrates), detergents (especially non-ionic detergents), TFA (&gt;0.10%) and other additives in the samples.<\/p>\n\n\n\n<p>Centrifuge the samples before analysis; undissolved particles\/precipitates in a sample can clog and reduce the life of the chromatography column.<\/p>\n\n\n\n<p>The Mass Spectrometry Service Center Staff are happy to answer any questions regarding sample preparation.<\/p>\n\n    <\/div><\/section>\n\n\n<h2 class=\"wp-block-heading  wsu-heading--style-marked\">Contact<\/h2>\n\n\n<div \n\tclass=\"wsu-people-list 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Area\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-website-link-text=\"Website\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-profile-link=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-headingTag=\"h2\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-columns=\"3\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-photo-size=\"medium\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-photo-srcset=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-filters=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-only-show-selected-term-values=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-exclude-term-values=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-include-term-values=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-directory-filter-label=\"Filter by Area\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-directory-filter-terms=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-category-filter-label=\"Filter by Category\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-category-filter-terms=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-classification-filter-label=\"Filter by Classification\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-classification-filter-terms=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-location-filter-label=\"Filter by Location\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-location-filter-terms=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-organization-filter-label=\"Filter by Organization\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-organization-filter-terms=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-search-filter-label=\"Type to search\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-tag-filter-label=\"Filter by Tag\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-tag-filter-terms=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-directory=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-exclude-child-directories=\"1\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-show-profile=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-indexProfiles=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-use-custom-profile-link=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-use-organization=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-custom-profile-link=\"\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-base-url=\"https:\/\/people.wsu.edu\"\n\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\tdata-children=\"\"\n\t\t><\/div>\n","protected":false},"excerpt":{"rendered":"<p>About the Center Contact<\/p>\n","protected":false},"author":41121,"featured_media":0,"parent":4656,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"categories":[],"tags":[],"wsuwp_university_location":[],"wsuwp_university_org":[],"_links":{"self":[{"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/pages\/3933"}],"collection":[{"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/users\/41121"}],"replies":[{"embeddable":true,"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/comments?post=3933"}],"version-history":[{"count":17,"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/pages\/3933\/revisions"}],"predecessor-version":[{"id":8611,"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/pages\/3933\/revisions\/8611"}],"up":[{"embeddable":true,"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/pages\/4656"}],"wp:attachment":[{"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/media?parent=3933"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/categories?post=3933"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/tags?post=3933"},{"taxonomy":"wsuwp_university_location","embeddable":true,"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/wsuwp_university_location?post=3933"},{"taxonomy":"wsuwp_university_org","embeddable":true,"href":"https:\/\/spokane.wsu.edu\/research\/wp-json\/wp\/v2\/wsuwp_university_org?post=3933"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}