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Flow Cytometry

Flow Cytometry is a highly quantitative high throughput technique to accurately detect and quantitate various cell components at the single cell level.

Bio-Rad S3 Cell Sorter

This automated benchtop cell sorter is equipped with 2 lasers, 4 fluorescense detectors, and forward- and side-scatter detectors. Features of the unit include simplified instrument setup, reduced carryover, 2-way cell sorting, high speed and high purity, and intuitive interface.

Excitation LaserAcquisition Channel/FilterExample Fluorescent Proteins
488 nm
561 nm
Co-linear
FI1 – 525/30
FI2 – 586/25
FI3 – 615/25
FI4 – 655LP
FitC, AF488, GFP, PE, PE-TR,
PI, mCherry from 561nm, PerCEP, PE-cy7

Beckman Coulter Gallios

This unit is a research system that delivers analytical excellence by coupling extraordinary sensitivity resolution and dynamic range with high speed data collection. Along with excellent detection capabilities, the Gallios includes easy to use software and automation to facilitate superior performance of multi-color flow cytometry assays.

Excitation LaserAcquisition Channel/FilterExample Fluorescent Proteins
488 nm
638 nm
FI1 – 525/40
FI2 – 575/30
FI3 – 620/30
FI4 – 695/30
FI5 – 755LP
FI6 – 660/20
FitC, AF488, GFP, PE, PE-TR, PI, PerCP, PE-Cy5.5, PE-Cy7, PE-AF700, APC, AF647

Becton Dickinson Accuri C6

This unit is equipped with 2 lasers, 2 light scatter detectors, and 4 fluroescense detectors with optical filters optimized for the detrection pf FITC, PE, PerCP, APC. Data is digitally collected over a wide dynamic range of 7.2 decades, making all data available to users as needed.

Excitation LaserAcquisition Channel/FilterExample Fluorescent Proteins
488 nm
640 nm
FI1 – 510/15
530/30
FI2 – 585/40
540/20
FI3 – 670LP
610/20
FI4 – 675/25
GFP, FitC, AF488,
PE, PI, YFP (bleeds into 530),
PerCP, PE-cy5.5, PI, PE-TR, APC, AF647, AF700

Software

Each cytometer has its own acquisition software, specifically designed to optimally run the machine and acquire data. All files can be saved as general format FCS files and further analyzed with Beckman Coulter Kaluza analysis software available for use in the CORE.

Training and Scheduling Use

Contact the Research Supervisor to arrange training. Training is for instrument use only. Please do not bring experimental samples to training without prior approval.

Use of the CORE facility is primarily for trained users although the Research Supervisor may be requested to operate the systems. Schedule planned usage on the calendars located by each piece of equipment.

Rates (Effective January 1, 2019)

EquipmentWSUNon-WSU
Galios$45/hr$50/hr
Accuri$40/hr$45/hr
S3 Cell Sorter$100/hr$110/hr
Computer Use Only$5/hr$6/hr
Technical Support$45/hr$50/hr
Training$45/hr$50/hr
Misuse FeeTo Cover Cost of Cleaning/RepairTo Cover Cost of Cleaning/Repair