Illumina Sequencing
Workflow of Typical Sequencing Projects
Pricing information and options
Note: As a reminder, it is best practice to plan for a 3 to 5 percent yearly rate increase when proposing studies and submitting grants.
Library Preparation
(all prices are per sample)
| Application Type | Examples of Reagents/Kits | Price |
|---|---|---|
| RNA-seq Library/mRNA | TruSeq RNA Prep v2 | $168 |
| RNA-seq Library/Total RNA | TruSeq Stranded Total with Ribo-zero | $221 |
| small RNA Library | TruSeq small RNA | $207 |
| Low Input RNA (10 pg) Library | SMARTer | $290 |
| ChIP-seq Library | TruSeq ChIP | $184 |
| Whole Genome Library | TruSeq Nano | $141 |
| Exome Library | Nextera Rapid Capture Exome | $256 |
| Methylation Library | EpiGome Methyl-Seq | $200 |
| Moleculo Library | Truseq Synthetic Long-Read DNA Library Prep Kit; TruSeq Synthetic Long-Read DNA Barcode Kit | $1,828 |
| Amplicon-based Library | Please inquire | Please inquire |
| De Novo Sequencing Library | Please inquire | Please inquire |
| Other Libraries | Please inquire | Please inquire |
Quant/QC of Libraries and Nucleic Acids
| Application Type | Reagents | Price |
|---|---|---|
| Library Quant by qPCR (per sample) | Kapa Library Quantification Kit | $30 |
| DNA/RNA Quant Qubit (per sample) | Qubit Assay Reagents | $5 |
| Library QC (per sample) | Fragment Analyzer Reagents | $10 |
Illumina HiSeq 2500 Sequencing
| High Output Run 200 million reads per lane | Unit | Price |
|---|---|---|
| Single Read 50 bp | Lane | $993 |
| Single Read 100 bp | Lane | $1,284 |
| Paired-End 50 bp | Lane | $1,614 |
| Paired-End 100 bp | Lane | $2,140 |
| Any configuration | Flow Cell (8 Lanes) | 10% Discount |
| Rapid Run 250 million reads per run |
||
|---|---|---|
| Single Read 50 bp | run | $2,187 |
| Single Read 100 bp | run | $2,685 |
| Single Read 250 bp | run | $5,479 |
| Paired-End 50 bp | run | $3,250 |
| Paired-End 100 bp | run | $4,261 |
| Paired-End 250 bp | run | $7,198 |