Recommended Biocontainment Levels for Cell Sorting
| Biocontainment for cell sorting | Exempt BSL-1 | BSL-1 | BSL-2 | BSL-2 Enhanced |
|---|---|---|---|---|
| Examples of cells | Wild type cells form murine or other non-human or non-primate species: 1) NOT exposed to any microbial agent 2) NOT genetically modified 3) Not fixed with paraformaldehyde/ formalin fixation | Cells from murine or other non-human or non-primate species 1) NOT been exposed to any microbial agent but have been genetically modified using non-viral methods (e.g. cells from transgenic animals or cells treated with nucleic acids) 2) Not fixed with paraformaldehyde or formalin fixation | 1) Unfixed human cells NOT exposed to any microbial agent and NOT been genetically modified 2) Unfixed cells from non-human primates that have been genetically modified/transduced with replication-deficient lentiviral or retroviral vector(s) or exposed to microbial agents (e.g. viral, bacterial, fungal, protozoan, parasitic) 3) Any source with paraformaldehyde or formalin fixation | Unfixed human cells that have been genetically modified/transduced with replication-deficient lentiviral or retroviral vector(s) or exposed to microbial agents (e.g. viral, bacterial, fungal, protozoan, parasitic) |