Flow Cytometry
Flow cytometry is a highly quantitative high throughput technique to accurately detect and quantitate various cell components at the single-cell level.
Training and Scheduling Use
Contact the research supervisor to arrange training. Training is for instrument use only. Please do not bring experimental samples to training without prior approval.
Use of the flow cytometry facility is primarily for trained users, though the research supervisor may be requested to operate the systems. You can schedule planned usage on the calendars located by each piece of equipment.
Equipment
Beckman Coulter Gallios
This unit is a research system that delivers analytical excellence by coupling extraordinary sensitivity resolution and dynamic range with high-speed data collection. Along with excellent detection capabilities, the Gallios includes easy to use software and automation to facilitate superior performance of multi-color flow cytometry assays.
| Excitation Laser | Acquisition Channel/Filter | Example Fluorescent Proteins |
|---|---|---|
| 488 nm 638 nm | FI1 – 525/40 FI2 – 575/30 FI3 – 620/30 FI4 – 695/30 FI5 – 755LP FI6 – 660/20 | FitC, AF488, GFP, PE, PE-TR, PI, PerCP, PE-Cy5.5, PE-Cy7, PE-AF700, APC, AF647 |
Software
Each cytometer has its own acquisition software, specifically designed to optimally run the machine and acquire data. All files can be saved as general format FCS files and further analyzed with Beckman Coulter Kaluza analysis software available for use in the CORE.
SONY MA900 Cell Sorter
The Sony MA900 is a powerful, easy to use benchtop cell sorter including a patented microfluidic chip-based design. MA900 has 4-laser (488 nm, 405 nm, 561 nm, and 638 nm) on two beam spots. It supports 12 fluorescence parameters and 4-way sorting. The MA900 offers 70 μm, 100 μm and 130 μm nozzle sizes that meet the needs of most sorting applications. Sort devices supported include 2-way and 4-way tubes as well as 6-, 12-, 24-, 48-, 96-, and 384-well plates.
